Funding Information
NIH / FDA
Platform for Rapid Expression, Purification, and Analysis of Patient-Specific Gene Therapy Products (September 2021)
Current
Bio-Rad
Custom Affinity Resins for AAV Purification (August 2022)
Current
Merck and Co.
Peptide-Based Adsorbents for the Purification of Viral Vaccines (September 2022)
Current
Merck KGaA
Evaluation of chromatographic adsorbents for viral vector purification and identification of optimal operating conditions (January 2023)
Current
NIIMBL
Modernizing clearance and quantification of host-cell proteins in mAb manufacture (September 2023)
Current
LigaTrap Technologies LLC
Developing continuous processes for biologics (March 2023)
Current
Triangle Universities Center for Advanced Studies, Inc.
An Academic-Industrial Partnership in the Research Triangle to Forge the Next-Generation Biomanufacturing of Gene Therapy Products (August 2023)
Current
Scholarly Output
Sobhana A. Sripada, Mahshid Hosseini, Srivatsan Ramesh, Junhyeong Wang, Kimberly Ritola, Stefano Menegatti and Michael Daniele; “Advances and Opportunities in Process Analytical Technologies in Viral Vector Manufacturing”. (Link)
Sobhana A. Sripada, ShriArjun Shastry, Eduardo Barbieri, Elena Wuestenhagen, Oliver Rammo, Michael Schulte, Michael Daniele, Stefano Menegatti; “Multiangle light scattering as a Lentivirus Process Analytical Technology”. (Link)
Jacob LeBarre, Scott H Altern, Wenning Chu, Andrew J Kocot, Eduardo Barbieri, Steven Cramer, Stefano Menegatti et. al., “Mixed-mode size-exclusion silica resin for polishing human antibodies in flow-through mode.” (Link)
Eduardo Barbieri, Eduardo, Gina N. Mollica, Brandyn Moore, Sobhana A. Sripada, Shriarjun Shastry, Ryan Kilgore, Casee M. Loudermilk, Zachary H. Whitacre, Katie M. Kilgour, Elena Wuestenhagen, Annika Aldinger, Heiner Graalfs, Oliver Rammo, Michael Schulte, Thomas Johnson, Michael A. Daniele, Stefano Menegatti, “Peptide ligands targeting the vesicular stomatitis virus G (VSV-G) protein for the affinity purification of lentivirus particles.” (Link).
ShriArjun Shastry, S., Wenning Chu, Eduardo Barbieri, Paul Greback-Clarke, William Smith, Christopher Cummings, Arianna Minzoni, Jennifer Pancorbo, Gary Gilleskie, Kimberly Ritola, Michael Daniele, and Stefano Menegatti, “Rational design and experimental evaluation of peptide ligands for the purification of adeno-associated viruses via affinity chromatography.” (2023, Link).
Sobhana A. Sripada, Driss Elhanafi, Leonard B. Collins, Taufika I. Williams, Marina Y. Linova, John M. Woodley, Cristiana Boi, Stefano Menegatti, “Pseudo-affinity capture of K. phaffii host cell proteins in flow-through mode: Purification of protein therapeutics and proteomic study”, Separation and Purification Technology, Volume 326, 2023, 124777, ISSN 1383-5866, (Link).
Ryan Kilgore, Arianna Minzoni; Shriarjun Shastry; Will Smith; Eduardo Barbieri; Yuxuan Wu; Jacob P. LeBarre; Wenning Chu; Juliana O’Brien; Stefano Menegatti*. “The downstream bioprocess toolbox for therapeutic viral vectors.” Journal of Chromatography A, August 28,2023 (Link).
Christopher Sharkey, Jack Twiddy, Kaila L. Peterson, Angelica F. Aroche, Stefano Menegatti* and M. Daniele.* “Towards Electrochemical Control of pH for Regeneration of Biosensors.”accepted for publication by IEEE BioSensors, June 2, 2023.
Junhyeong Wang, Mahshid Hosseini, Eduardo Barbieri, Shriarjun Shastry, Wenning Chu, Stefano Menegatti, and Michael Daniele, “Toward the Quantification of adeno-Associated Virus Titer by Electrochemical Impedance Spectroscopy”, IEEE BioSensors, June 2, 2023 (Link).
Ka Zhang, Eduardo Barbieri, Jacob LeBarre, Shahid Rameez, Sigma Mostafa, Stefano Menegatti. “Peptonics: a new family of cell-protecting surfactants for the recombinant expression of therapeutic proteins in mammalian cell cultures.” (Link).
Jinxin Fan, Barbieri E, Shastry S, Menegatti S, Boi C, Carbonell RG. Purification of Adeno-Associated Virus (AAV) Serotype 2 from Spodoptera frugiperda (Sf9) Lysate by Chromatographic Nonwoven Membranes. Membranes (Basel) 2022. (Link)
Maddocks, Grace M., Kaila L. Peterson, McKenna L. Downey, Bang Hyun Lee, Joseph H. Lavoie, Stefano Menegatti, and Michael Daniele. “Aptasensor for Detection of Influenza-A in Human Saliva.” In 2022 44th Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC), pp. 1262-1265. IEEE, 2022.
Jinxin Fan, Sobhana A. Sripada, Dan N. Pham, Marina Y. Linova, John M. Woodley, Stefano Menegatti, Cristiana Boi, Ruben G. Carbonell, “Purification of a monoclonal antibody using a novel high-capacity multimodal cation exchange nonwoven membrane.” Separation and Purification Technology 317 (2023): 123920 (Link).
Chu, Wenning, Shriarjun Shastry, Eduardo Barbieri, Raphael Prodromou, Paul Greback-Clarke, Will Smith, Brandyn Moore, Ryan Kilgore, Christopher Cummings, Jennifer Pancorbo, Gary Gilleskie, Michael A. Daniele, and Stefano Menegatti, “Peptide ligands for the affinity purification of adeno-associated viruses from HEK 293 cell lysates.” Biotechnology and Bioengineering (Link).
US-PRV 63/484,300 Affinity Ligands for Purification of AAVs from HEK293 Lysate Provisional – Filed US-PRV 63/501,785 Peptide mimetics of AAV-R and aAAV mAbs as Ligands for Affinity Purification of AAVs Provisional – Filed TBD Affinity Ligands for Purification of Lentivirus Provisional – Submitted, Non-Commercial License with Millipore Sigma